USP7 Recombinant Rabbit Monoclonal Antibody [JB80-36]
cat.: ET7107-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JB80-36
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 128 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human USP7 aa 80-180 / 1,102.
Positive control: Wild-type A375 whole cell lysates, 293T cell lysates, HeLa cell lysates, rat brain tissue, human tonsil tissue, mouse colon tissue, PC-3M.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50
1:50-1:100
Uniprot #: SwissProt: Q93009 Human | Q6A4J8 Mouse | Q4VSI4 Rat
Alternative names: Deubiquitinating enzyme 7 HAUSP Herpes virus associated ubiquitin specific protease Herpesvirus-associated ubiquitin-specific protease TEF 1 tef-1 TEF1 Ubiquitin carboxyl terminal hydrolase 7 Ubiquitin carboxyl-terminal hydrolase 7 Ubiquitin specific peptidase 7 (herpes virus associated) Ubiquitin specific peptidase 7 Ubiquitin specific peptidase 7 herpes virus associated Ubiquitin specific processing protease 7 Ubiquitin specific protease 7 (herpes virus associated) Ubiquitin specific protease 7 Ubiquitin specific protease 7 herpes virus associated Ubiquitin thioesterase 7 Ubiquitin thiolesterase 7 Ubiquitin-specific-processing protease 7 UBP 7 UBP-7 UBP7 UBP7_HUMAN USP 7 usp-7 Usp7 VMW110-ASSOCIATED PROTEIN, 135-KD
Images
ET7107-53_1.jpg Fig1: All lanes: Western blot analysis of USP7 with anti-USP7 antibody [JB80-36] (ET7107-53) at 1:1,000 dilution.
Lane 1: Wild-type A375 whole cell lysate (20 µg).
Lane 2: USP7 knockout A375 whole cell lysate (20 µg).

ET7107-53 was shown to specifically react with USP7 in wild-type A375 cells. No band was observed when USP7 knockout sample was tested. Wild-type and USP7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-53, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7107-53_2.jpg Fig2: Western blot analysis of USP7 on different lysates with Rabbit anti-USP7 antibody (ET7107-53) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 128 kDa
Observed band size: 128 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7107-53_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-USP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-53_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-USP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-USP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-53_6.jpg Fig6: Flow cytometric analysis of USP7 was done on PC-3M cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.