Galectin 7 Recombinant Rabbit Monoclonal Antibody [JB38-11]
cat.: ET7107-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB38-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Galectin 7 aa 2-51 / 136. |
| Positive control: | Human skin tissue lysates, HUVEC, A431, human tonsil tissue, human skin tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Secreted. |
| Recommended Dilutions:
IF-Cell IHC-P WB IP |
1:50-1:200 1:100-1:300 1:500-1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: P47929 Human |
| Alternative names: | Gal-7 GAL7 Galectin 7B Galectin-7 HKL-14 Human keratinocyte lectin 14 Keratinocyte lectin 14 Lectin galactoside binding soluble 7 Lectin, galactoside binding, soluble, 7B LEG7_HUMAN LGALS7 LGALS7A LGALS7B P53 induced protein 1 p53-induced gene 1 protein Pi7 PIG1 TP53I1 |
Images
|
Fig1:
Western blot analysis of Galectin 7 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (ET7107-54, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 15 kDa Observed band size: 15 kDa |
|
Fig2: ICC staining of Galectin 7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of Galectin 7 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Galectin 7 antibody (ET7107-54) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-54) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Galectin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.