CD3G Recombinant Rabbit Monoclonal Antibody [JB38-29]
cat.: ET7107-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JB38-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD3G aa 133-182 / 182. |
| Positive control: | Mouse thymus tissue lysates, Jurkat cell lysates, human tonsil tissue, human spleen tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P09693 Human | P11942 Mouse |
| Alternative names: | CD3 gamma CD3-GAMMA CD3g CD3g antigen gamma polypeptide (TiT3 complex) CD3g antigen gamma polypeptide CD3g molecule gamma (CD3 TCR complex) CD3g molecule gamma CD3G_HUMAN FLJ17620 FLJ17664 FLJ79544 FLJ94613 MGC138597 T cell antigen receptor complex gamma subunit of T3 T-cell receptor T3 gamma chain T-cell surface glycoprotein CD3 gamma chain T3G |
Images
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Fig1:
Western blot analysis of CD3G on different lysates with Rabbit anti-CD3G antibody (ET7107-55) at 1/500 dilution. Lane 1: Mouse thymus tissue lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-55) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of CD3G was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-55, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.