Galectin 8 Recombinant Rabbit Monoclonal Antibody [JB85-35]
cat.: ET7107-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB85-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Galectin 8 aa 1-50 / 317. |
| Positive control: | Mouse testis tissue lysate, rat testis tissue lysate, A431, HepG2, human kidney tissue, human liver tissue, human colon carcinoma tissue, PC-3M. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:500-1:1,000 1:400-1:800 1:50-1:200 |
| Uniprot #: | SwissProt: O00214 Human | Q9JL15 Mouse | Q62665 Rat |
| Alternative names: | Gal 8 Gal-8 Gal8 Galectin-8 galectin-8g Lectin galactoside binding soluble 8 LEG8_HUMAN LGAL S8 Lgals8 PCTA 1 PCTA-1 PCTA1 Po66 carbohydrate binding protein Po66 carbohydrate-binding protein Po66 CBP Po66-CBP Prostate carcinoma tumor antigen 1 |
Images
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Fig1:
Western blot analysis of Galectin 8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.