Galectin 8 Recombinant Rabbit Monoclonal Antibody [JB85-35]
cat.: ET7107-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB85-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Galectin 8 aa 1-50 / 317. |
| Positive control: | PANC-1 cell lysate,Mouse testis tissue lysate, rat testis tissue lysate, A431, HepG2, human kidney tissue, human liver tissue, human colon carcinoma tissue, PC-3M. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:5,000 1:400-1:800 1:50-1:200 |
| Uniprot #: | SwissProt: O00214 Human | Q9JL15 Mouse | Q62665 Rat |
| Alternative names: | Gal 8 Gal-8 Gal8 Galectin-8 galectin-8g Lectin galactoside binding soluble 8 LEG8_HUMAN LGAL S8 Lgals8 PCTA 1 PCTA-1 PCTA1 Po66 carbohydrate binding protein Po66 carbohydrate-binding protein Po66 CBP Po66-CBP Prostate carcinoma tumor antigen 1 |
Images
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Fig1:
Western blot analysis of Galectin 8 on different lysates with Rabbit anti-Galectin 8 antibody (ET7107-56) at 1/5,000 dilution. Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Cell lysates at 10 µg/Lane,tissue lysates at 10 µg/Lane Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7107-56, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 42 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.