Proteasome 20S C2 Recombinant Rabbit Monoclonal Antibody [JB89-30]
cat.: ET7107-59
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JB89-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Proteasome 20S C2 aa 214-263 / 263. |
| Positive control: | PC-3M cell lysate, K562 cell lysate, rat testis tissue lysate, mouse testis tissue lysate, mouse colon tissue lysate, human pancreas tissue, rat testis tissue, mouse cerebellum tissue, human liver cancer tissue, K562. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P25786 Human | Q9R1P4 Mouse | P18420 Rat |
| Alternative names: | 30 kDa prosomal protein HC 2 HC2 Macropain subunit C2 Macropain subunit nu MGC14542 MGC14575 MGC14751 MGC1667 MGC21459 MGC22853 MGC23915 Multicatalytic endopeptidase complex subunit C2 NU PROS 30 PROS-30 PROS30 Proteasome (prosome macropain) subunit alpha type 1 Proteasome alpha 1 subunit Proteasome component C2 Proteasome nu chain Proteasome subunit alpha type 1 Proteasome subunit alpha type I Proteasome subunit alpha type-1 Proteasome subunit nu Protein P30 33K PSA1_HUMAN PSC 2 PSC2 PSMA 1 psmA1 |
Images
|
Fig1:
Western blot analysis of Proteasome 20S C2 on different lysates with Rabbit anti-Proteasome 20S C2 antibody (ET7107-59) at 1/500 dilution. Lane 1: PC-3M cell lysate (10 µg/Lane) Lane 2: K562 cell lysate (10 µg/Lane) Lane 3: Rat testis tissue lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (20 µg/Lane) Lane 5: Mouse colon tissue lysate (20 µg/Lane) Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-59) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin. |
|
Fig6: Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.