Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JB93-32 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 57 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Cytochrome P450 17A1 aa 81-240 / 508. |
Positive control: | HepG2, Hela, SH-SY5Y, human breast tissue, human kidney tissue, mouse kidney tissue, rat testis tissue lysate. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P05093 Human | P27786 Mouse | P11715 Rat |
Alternative names: | 20 lyase CP17A_HUMAN CPT7 CYP17 CYP17A1 CYPXVII Cytochrome P450 17A1 Cytochrome P450 family 17 Cytochrome P450 family 17 subfamily A polypeptide 1 Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia Cytochrome p450 XVIIA1 Cytochrome P450-C17 Cytochrome P450c17 OTTHUMP00000020382 P450 C17 P450c17 S17AH Steroid 17 alpha hydroxylase/17,20 lyase Steroid 17 alpha monooxygenase Steroid 17-alpha-hydroxylase/17 Steroid 17-alpha-monooxygenase |
Fig1: ICC staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Cytochrome P450 17A1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin. | |
Fig7: Flow cytometric analysis of SH-SY5Y cells with Cytochrome P450 17A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. | |
Fig8:
Western blot analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody (ET7107-61) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-61) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |