CYP17A1 Recombinant Rabbit Monoclonal Antibody [JB93-32]
cat.: ET7107-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JB93-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CYP17A1 aa 81-240 / 508. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Rat brain tissue lysate, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P05093 Human | P27786 Mouse | P11715 Rat |
| Alternative names: | 20 lyase CP17A_HUMAN CPT7 CYP17 CYP17A1 CYPXVII Cytochrome P450 17A1 Cytochrome P450 family 17 Cytochrome P450 family 17 subfamily A polypeptide 1 Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia Cytochrome p450 XVIIA1 Cytochrome P450-C17 Cytochrome P450c17 OTTHUMP00000020382 P450 C17 P450c17 S17AH Steroid 17 alpha hydroxylase/17,20 lyase Steroid 17 alpha monooxygenase Steroid 17-alpha-hydroxylase/17 Steroid 17-alpha-monooxygenase |
Images
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Fig1:
Western blot analysis of CYP17A1 on different lysates with Rabbit anti-CYP17A1 antibody (ET7107-61) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CYP17A1 antibody (ET7107-61) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-61) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CYP17A1 antibody (ET7107-61) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-61) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.