Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33]
cat.: ET7107-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB95-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Glutathione Synthetase aa 301-420 / 474. |
| Positive control: | HeLa cell lysate, Human liver tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytosol. Extracellular region or secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P48637 Human | P51855 Mouse | P46413 Rat |
| Alternative names: | epididymis secretory sperm binding protein Li 64p epididymis secretory sperm binding protein Li 88n Glutathione synthase Glutathione synthetase GSH S GSH synthetase GSH-S GSHB_HUMAN GSHS GSS HEL-S-64p HEL-S-88n MGC14098 OTTHUMP00000030711 |
Images
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Fig1:
Western blot analysis of Glutathione Synthetase on different lysates with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glutathione Synthetase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-62) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Glutathione Synthetase on different lysates with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Human liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.