FKBP51 Recombinant Rabbit Monoclonal Antibody [JB95-39]
cat.: ET7107-64
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JB95-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human FKBP51 aa 400-440.
Positive control: Daudi cell lysate, Hela cell lysate, human tonsil tissue, human kidney tissue, rat lung tissue, Daudi.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
1:500-1:1,000
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q13451 Human | Q5U2T9 Rat
Alternative names: 51 kDa FK506 binding protein 5 51 kDa FK506 binding protein 51 kDa FK506-binding protein 51 kDa FKBP 54 kDa progesterone receptor associated immunophilin 54 kDa progesterone receptor-associated immunophilin AIG 6 AIG6 Androgen regulated protein 6 Androgen-regulated protein 6 FF1 antigen FK506 binding protein 5 FK506-binding protein 5 FKBP 5 FKBP 51 FKBP 54 FKBP-5 FKBP-51 FKBP5 FKBP5_HUMAN FKBP54 HSP90 binding immunophilin HSP90-binding immunophilin MGC111006 OTTHUMP00000016268 P54 Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP5 Peptidylprolyl cis trans isomerase PPIase PPIase FKBP5 Ptg 10 Ptg10 Rotamase T cell FK506 binding protein
Images
ET7107-64_1.jpg Fig1: Western blot analysis of FKBP51 on different lysates with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si FKBP51 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-64_2.jpg Fig2: Western blot analysis of FKBP51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: Hela cell lysate
ET7107-64_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-64_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-64_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-64_6.jpg Fig6: Flow cytometric analysis of FKBP51 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.