ET7107-64

FKBP51 Recombinant Rabbit Monoclonal Antibody [JB95-39]

cat.: ET7107-64

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JB95-39

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 51 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human FKBP51 aa 400-440.
Positive control:	HCT 116 cell lysate, K-562 cell lysates, K-562, human kidney tissue.
Subcellular location:	Nucleus, Cytoplasm.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000 1:100 1:200 1:1,000
Uniprot #:	SwissProt: Q13451 Human \| Q5U2T9 Rat
Alternative names:	51 kDa FK506 binding protein 5 51 kDa FK506 binding protein 51 kDa FK506-binding protein 51 kDa FKBP 54 kDa progesterone receptor associated immunophilin 54 kDa progesterone receptor-associated immunophilin AIG 6 AIG6 Androgen regulated protein 6 Androgen-regulated protein 6 FF1 antigen FK506 binding protein 5 FK506-binding protein 5 FKBP 5 FKBP 51 FKBP 54 FKBP-5 FKBP-51 FKBP5 FKBP5_HUMAN FKBP54 HSP90 binding immunophilin HSP90-binding immunophilin MGC111006 OTTHUMP00000016268 P54 Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP5 Peptidylprolyl cis trans isomerase PPIase PPIase FKBP5 Ptg 10 Ptg10 Rotamase T cell FK506 binding protein

Images

Fig1: Western blot analysis of FKBP51 on different lysates with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si FKBP51 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of FKBP51 on K-562 cell lysates with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunocytochemistry analysis of K-562 cells labeling FKBP51 with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
	Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-FKBP51 antibody (ET7107-64) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-64) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Flow cytometric analysis of K-562 cells labeling FKBP51. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-64, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.