Desmoglein 2 Recombinant Rabbit Monoclonal Antibody [JB96-37]
cat.: ET7107-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB96-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Desmoglein 2 aa 961-1,118 / 1,118. |
| Positive control: | A431 cell lysate, SiHa cell lysate, rat heart tissue, human colon cancer tissue, human prostate tissue, human placenta tissue, mouse small intestine tissue, A431. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q14126 Human | O55111 Mouse | F1LYX9 Rat |
| Alternative names: | ARVC 10 ARVC10 ARVD 10 ARVD10 Cadherin family member 5 CDHF 5 CDHF5 CMD1BB Desmoglein 2 Desmoglein-2 Desmoglein2 DSG 2 DSG2 DSG2_HUMAN HDGC HDGC included Human Desmoglein colon MGC117034 MGC117036 MGC117037 |
Images
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Fig1:
Western blot analysis of Desmoglein 2 on different lysates with Rabbit anti-Desmoglein 2 antibody (ET7107-65) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 122 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-65) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.