MCM3 Recombinant Rabbit Monoclonal Antibody [JB99-35]
cat.: ET7107-66
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB99-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MCM3 aa 1-50 / 808. |
| Positive control: | Mouse thymus tissue lysate, NIH-3T3 cell lysates, Hela cell lysates, 293T, HepG2, SH-SY-5Y, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562. |
| Subcellular location: | Chromosome, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P25205 Human | P25206 Mouse | D3ZFP4 Rat |
| Alternative names: | Cervical cancer proto oncogene 5 DNA polymerase alpha holoenzyme associated P1 DNA polymerase alpha holoenzyme associated protein P1 DNA polymerase alpha holoenzyme-associated protein P1 DNA replication factor MCM3 DNA replication licensing factor mcm3 HCC 5 HCC5 hRlf beta subunit Human cervical cancer proto oncogene 5 MCM 3 mcm3 MCM3 minichromosome maintenance deficient 3 MCM3_HUMAN MGC1157 Minichromosome maintenance complex component 3 Minichromosome maintenance deficient 3 Minichromosome maintenance protein 3 P1 h P1 MCM3 P1 Protein P1-MCM3 P1.h p102 P102 protein Replication licensing factor beta subunit RLF beta subunit RLF subunit beta RLFB |
Images
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Fig1: Western blot analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell lysates (2) and Hela cell lysates (3) using anti-MCM3 antibody at 1/500 dilution. |
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Fig2: ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.