MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]
cat.: ET7107-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JB40-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MYBBP1A aa 1,051-1,100 / 1,328. |
| Positive control: | A549 cell lysates, HeLa. |
| Subcellular location: | Cytoplasm, Nucleus, nucleolus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9BQG0 Human |
| Alternative names: | AL024407 AU019902 cb486 FLJ37886 MBB1A_HUMAN MYB binding protein (P160) 1a myb binding protein (P160) 1a like Myb-binding protein 1A Mybbp1a nuclear protein P160 P160 p160MBP p53 activated protein 2 p67MBP PAP2 PAR interacting protein RP23 48A2.3 sb:cb486 |
Images
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Fig1: Western blot analysis of MYBBP1A on A549 cell lysate using anti-MYBBP1A antibody at 1/500 dilution. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling MYBBP1A with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling MYBBP1A. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-67, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.