Frizzled 8 Recombinant Rabbit Monoclonal Antibody [JB40-16]
cat.: ET7107-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB40-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Frizzled 8 aa 561-694 / 694. |
| Positive control: | Mouse lung tissue lysates, human uterus tissue, human kidney tissue. |
| Subcellular location: | Golgi apparatus. Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H461 Human | Q61091 Mouse |
| Alternative names: | Frizzled 8 seven transmembrane spanning receptor frizzled 8, seven transmembrane spanning receptor Frizzled family receptor 8 frizzled homolog 8 (Drosophila) Frizzled homolog 8 Frizzled-8 FZ 8 Fz-8 FZ8 FZD 8 FZD8 FZD8_HUMAN hFZ 8 hFz8 HGNC4046 Homolog of Drosophila Frizzled 8 |
Images
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Fig1: Western blot analysis of Frizzled 8 on mouse lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Frizzled 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Frizzled 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.