Frizzled 8 Recombinant Rabbit Monoclonal Antibody [JB40-16]
cat.: ET7107-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB40-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Frizzled 8 aa 561-694 / 694. |
| Positive control: | Mouse lung tissue lysates, human uterus tissue, human kidney tissue. |
| Subcellular location: | Golgi apparatus. Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H461 Human | Q61091 Mouse |
| Alternative names: | Frizzled 8 seven transmembrane spanning receptor frizzled 8, seven transmembrane spanning receptor Frizzled family receptor 8 frizzled homolog 8 (Drosophila) Frizzled homolog 8 Frizzled-8 FZ 8 Fz-8 FZ8 FZD 8 FZD8 FZD8_HUMAN hFZ 8 hFz8 HGNC4046 Homolog of Drosophila Frizzled 8 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Frizzled 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Frizzled 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of Frizzled 8 on different lysates with Rabbit anti-Frizzled 8 antibody (ET7107-68) at 1/1,000 dilution. Lane 1: mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7107-68, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 73 kDa Observed band size: 73 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.