CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33]
cat.: ET7107-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB40-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 165 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CPS1 aa 82-204 / 1,500. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, mouse small intestine tissue lysate, rat liver tissue lysate, HepG2, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:500-1:1,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P31327 Human | Q8C196 Mouse | P07756 Rat |
| Alternative names: | Carbamoyl phosphate synthase [ammonia] Carbamoyl phosphate synthase [ammonia] mitochondrial Carbamoyl phosphate synthase Carbamoyl phosphate synthetase 1 Carbamoyl phosphate synthetase 1 mitochondrial Carbamoyl phosphate synthetase I Carbamoyl-phosphate synthase [ammonia] Carbamoyl-phosphate synthetase I Carbamoylphosphate synthase Carbamoylphosphate synthetase 1 Carbamoylphosphate synthetase I CPS 1 Cps1 CPSase 1 CPSase I CPSASE1 CPSM_HUMAN mitochondrial MS738 |
Images
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Fig1:
Western blot analysis of CPS1 on different lysates with Rabbit anti-CPS1 antibody (ET7107-69) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Mouse small intestine tissue lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 150 kDa Exposure time: Lane 1-2: 25 seconds; Lane 3-4: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of CPS1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 2 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.