CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33]
cat.: ET7107-69
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: JB40-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 165 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CPS1 aa 82-204 / 1,500.
Positive control: SiHa cell lysates, HepG2, human liver tissue, mouse small intestine tissue, rat liver tissue.
Subcellular location: Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:500-1:1,000
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P31327 Human | Q8C196 Mouse | P07756 Rat
Alternative names: Carbamoyl phosphate synthase [ammonia] Carbamoyl phosphate synthase [ammonia] mitochondrial Carbamoyl phosphate synthase Carbamoyl phosphate synthetase 1 Carbamoyl phosphate synthetase 1 mitochondrial Carbamoyl phosphate synthetase I Carbamoyl-phosphate synthase [ammonia] Carbamoyl-phosphate synthetase I Carbamoylphosphate synthase Carbamoylphosphate synthetase 1 Carbamoylphosphate synthetase I CPS 1 Cps1 CPSase 1 CPSase I CPSASE1 CPSM_HUMAN mitochondrial MS738
Images
ET7107-69_1.jpg Fig1: Western blot analysis of CPS1 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7107-69_2.jpg Fig2: ICC staining of CPS1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-69_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CPS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-69_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-CPS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-69_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 2 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.