SF2 Recombinant Rabbit Monoclonal Antibody [JB40-76]
cat.: ET7107-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JB40-76
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SF2 aa 1-50 / 248.
Positive control: Mouse heart tissue lysate, mouse liver tissue lysate, K562 cell lysate, A431, human lung carcinoma tissue, human tonsil tissue, human breast tissue, mouse colon tissue, rat epididymis tissue, K562.
Subcellular location: Cytoplasm, Nucleus, Spliceosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q07955 Human | Q6PDM2 Mouse | D4A9L2 Rat
Alternative names: Alternative splicing factor 1 Alternative-splicing factor 1 arginine/serine-rich 1 ASF 1 ASF ASF-1 ASF1 FLJ53078 MGC5228 P33 subunit Pre mRNA splicing factor SF2 P33 subunit pre-mRNA-splicing factor SF2 Serine/arginine-rich splicing factor 1 SF2 SF2P33 SFRS1 Splicing factor 2 alternate splicing factor Splicing factor 2 Splicing factor Splicing factor arginine/serine rich 1 SR Splicing factor 1 SRp30a srsf1 SRSF1_HUMAN
Images
ET7107-70_1.jpg Fig1: Western blot analysis of SF2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: K562 cell lysate
ET7107-70_2.jpg Fig2: ICC staining of SF2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-70_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-SF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-70_8.jpg Fig8: Flow cytometric analysis of SF2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-70, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.