RRM1 Recombinant Rabbit Monoclonal Antibody [JB09-43]
cat.: ET7107-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JB09-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RRM1 aa 651-792 / 792. |
| Positive control: | Mouse thymus tissue lysate, A431 cell lysate, human colon cancer tissue, human lymph node tissue, A431. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: P23921 Human | P07742 Mouse |
| Alternative names: | R1 Ribonucleoside diphosphate reductase large subunit Ribonucleoside diphosphate reductase M1 chain Ribonucleoside diphosphate reductase subunit M1 Ribonucleoside reductase, large subunit Ribonucleoside-diphosphate reductase large subunit Ribonucleoside-diphosphate reductase subunit M1 Ribonucleotide reductase large chain Ribonucleotide reductase large subunit Ribonucleotide reductase M1 Ribonucleotide reductase M1 polypeptide Ribonucleotide reductase R1 subunit Ribonucleotide reductase, M1 subunit RIR 1 RIR1 RIR1_HUMAN RR 1 RR1 RRM 1 RRM1 |
Images
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Fig1:
Western blot analysis of RRM1 on different lysates with Rabbit anti-RRM1 antibody (ET7107-72) at 1/500 dilution. Lane 1: Mouse thymus tissue lysate (20 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RRM1 antibody (ET7107-72) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-72) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-RRM1 antibody (ET7107-72) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-72) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of RRM1 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.