CD27 Recombinant Rabbit Monoclonal Antibody [JB40-98]
cat.: ET7107-73
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JB40-98
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Human Intracellular domain of CD27 aa 51-100 / 260.
Positive control: Raji cell lysate, mouse spleen tissue, K562, human tonsil tissue, rat spleen tissue, Daudi.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:500-1:1,000
1:200-1:1,000
1:50-1:200
Uniprot #: SwissProt: P26842 Human | P41272 Mouse
Entrez Gene: 500318 Rat
Alternative names: CD 27 CD27 CD27 antigen CD27 molecule CD27_HUMAN CD27L receptor LPFS2 MGC20393 OTTHUMP00000238557 S152 T cell activation antigen CD27 T cell antivation antigen S152 T-cell activation antigen CD27 T14 TNFRSF 7 TNFRSF7 TNFSF7 Tp 55 Tp55 Tumor necrosis factor receptor superfamily member 7
Images
ET7107-73_1.jpg Fig1: Western blot analysis of CD27 on different lysates with Rabbit anti-CD27 antibody (ET7107-73) at 1/2,000 dilution.

Lane 1: Raji cell lysate
Lane 2: MCF7 cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 50 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-73_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD27 antibody (ET7107-73) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-73_3.jpg Fig3: ICC staining of CD27 in K562 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-73_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD27 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-73_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD27 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.