CD27 Recombinant Rabbit Monoclonal Antibody [JB40-98]
cat.: ET7107-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB40-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human Intracellular domain of CD27 aa 51-100 / 260. |
| Positive control: | Raji cell lysate, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:5,000 |
| Uniprot #: | SwissProt: P26842 Human | P41272 Mouse Entrez Gene: 500318 Rat |
| Alternative names: | CD 27 CD27 CD27 antigen CD27 molecule CD27_HUMAN CD27L receptor LPFS2 MGC20393 OTTHUMP00000238557 S152 T cell activation antigen CD27 T cell antivation antigen S152 T-cell activation antigen CD27 T14 TNFRSF 7 TNFRSF7 TNFSF7 Tp 55 Tp55 Tumor necrosis factor receptor superfamily member 7 |
Images
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Fig1:
Western blot analysis of CD27 on different lysates with Rabbit anti-CD27 antibody (ET7107-73) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: MCF7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD27 antibody (ET7107-73) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD27 antibody (ET7107-73) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.