eEF1A1 Recombinant Rabbit Monoclonal Antibody [JB44-13]
cat.: ET7107-75
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JB44-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human eEF1A1 aa 413-462 / 462.
Positive control: Rat brain tissue tissue lysate, mouse skeletal muscle tissue lysate, mouse cerebellum tissue lysate, rat skin tissue lysate, Daudi cell lysate, HepG2, HUVEC, SH-SY5Y, rat skeletal muscle tissue, human tonsil tissue, human liver carcinoma tissue, human kidney tissue, mouse smooth muscle tissue, THP-1.
Subcellular location: Cell membrane, Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P68104 Human | P10126 Mouse | P62630 Rat
Alternative names: CCS 3 CCS3 Cervical cancer suppressor 3 chunp6927 CTCL tumor antigen EE1A1 EEF 1 EEF1A eEF1A-1 EEF1A1 EF-1-alpha-1 EF-Tu EF1A EF1a like protein EF1A1_HUMAN Elongation factor 1 alpha subunit Elongation factor 1-alpha 1 Elongation factor Tu Eukaryotic elongation factor 1 A-1 Eukaryotic translation elongation factor 1 alpha 1 Eukaryotic translation elongation factor 1 alpha 1 like 14 Glucocorticoid receptor AF 1 specific elongation factor GRAF 1EF HNGC:16303 ik:tdsubc_2a3 ik:tdsubc_2b3 LENG7 Leukocyte receptor cluster (LRC) member 7 Leukocyte receptor cluster member 7 Prostate tumor inducing protein 1 PTI1 tdsubc_2a3 Translation elongation factor 1 alpha 1 like 14 wu:fa91c07 wu:fa94b03 wu:fi13b09 xx:tdsubc_2a3 xx:tdsubc_2b3
Images
ET7107-75_1.jpg Fig1: Western blot analysis of eEF1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Rat skin tissue lysate
Lane 5: Daudi cell lysate
ET7107-75_2.jpg Fig2: ICC staining of eEF1A1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-75_3.jpg Fig3: ICC staining of eEF1A1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-75_4.jpg Fig4: ICC staining of eEF1A1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-75_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-75_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-75_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-eEF1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-75_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-75_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-75_10.jpg Fig10: Flow cytometric analysis of eEF1A1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-75, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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