MBD3 Recombinant Rabbit Monoclonal Antibody [JB61-47]
cat.: ET7107-78
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JB61-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MBD3 aa 160-291 / 291. |
| Positive control: | MCF-7 cell lysate, A549, NIH/3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Chromosome, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:5,000 1:50-1:200 1:50-1:200 1:50-1:500 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O95983 Human | Q9Z2D8 Mouse | F7EY92 Rat |
| Alternative names: | AI181826 AU019209 MBD 3 Mbd3 MBD3: methyl CpG binding domain protein 3 MBD3_HUMAN Methyl CpG binding domain protein 3 Methyl CpG binding protein MBD3 Methyl-CpG-binding domain protein 3 Methyl-CpG-binding protein MBD3 |
Images
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Fig1:
Western blot analysis of MBD3 on different lysates with Rabbit anti-MBD3 antibody (ET7107-78) at 1/5,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane) Lane 2: U-937 (Human acute monocytic leukemia cell) cell lysate (15 µg/Lane) Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (30 µg/Lane) Lane 6: Rat brainc tissue lysate (30 µg/Lane) Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7107-78, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 35 kDa |
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Fig2: ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining MBD3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MBD3 antibody (ET7107-78) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-78) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MBD3 antibody (ET7107-78) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-78) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.