ET7107-79

PAK2 Recombinant Rabbit Monoclonal Antibody [JB41-84]

cat.: ET7107-79

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey
Applications:	WB, IHC-P, FC, IF-Cell, IF-Tissue
Clonality:	Monoclonal
Clone number:	JB41-84

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 58 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within N-terminal Human PAK2 .
Positive control:	HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A549 cell lysate, human kidney tissue, human skeletal muscle tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue, rat skeletal muscle tissue, A431, SiHa.
Subcellular location:	Cytoplasm, Membrane, Nucleus.
Recommended Dilutions: WB IHC-P IF-Cell IF-Tissue FC	1:1,000-1:2,000 1:200-1:1,000 1:100-1:500 1:100-1:500 1:1,000
Uniprot #:	SwissProt: Q13177 Human \| Q8CIN4 Mouse \| Q64303 Rat
Alternative names:	C-t-PAK2 CB422 EC 2.7.11.1 Gamma PAK Gamma-PAK hPAK65 Kinase p21 (CDKN1A) activated kinase 2 p21 (CDKN1A)-activated kinase 2a p21 activated kinase 2 p21 protein (Cdc42/Rac)-activated kinase 2 p21 protein Cdc42 Rac activated kinase 2 p21-activated kinase 2 p21-activated kinase, 65-KD p21-activated protein kinase I p21CDKN1A activated kinase 2 p27 p34 p58 p65PAK PAK 2 PAK-2 PAK-2p34 Pak2 PAK2_HUMAN PAK65 PAKgamma S6 H4 kinase S6/H4 kinase Serine threonine protein kinase PAK 2 Serine/threonine protein kinase PAK 2

Images

Fig1: Western blot analysis of PAK2 on different lysates with Rabbit anti-PAK2 antibody (ET7107-79) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 12 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-79) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PAK2 on different lysates with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si PAK2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PAK2 antibody (ET7107-79) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-79) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: ICC staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig10: ICC staining PAK2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig11: Flow cytometric analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.