PAK2 Recombinant Rabbit Monoclonal Antibody [JB41-84]
cat.: ET7107-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JB41-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human PAK2 . |
| Positive control: | Mouse thymus tissue lysate, MCF-7 cell lysate, A431, SiHa, human colon cancer tissue, human liver tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue FC |
1:500-1:1,000 1:100-1:500 1:100-1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q13177 Human | Q8CIN4 Mouse | Q64303 Rat |
| Alternative names: | C-t-PAK2 CB422 EC 2.7.11.1 Gamma PAK Gamma-PAK hPAK65 Kinase p21 (CDKN1A) activated kinase 2 p21 (CDKN1A)-activated kinase 2a p21 activated kinase 2 p21 protein (Cdc42/Rac)-activated kinase 2 p21 protein Cdc42 Rac activated kinase 2 p21-activated kinase 2 p21-activated kinase, 65-KD p21-activated protein kinase I p21CDKN1A activated kinase 2 p27 p34 p58 p65PAK PAK 2 PAK-2 PAK-2p34 Pak2 PAK2_HUMAN PAK65 PAKgamma S6 H4 kinase S6/H4 kinase Serine threonine protein kinase PAK 2 Serine/threonine protein kinase PAK 2 |
Images
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Fig1:
Western blot analysis of PAK2 on different lysates with Rabbit anti-PAK2 antibody (ET7107-79) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PAK2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of PAK2 on mouse thymus tissue lysate (1) and MCF-7 cell lysate(2) using anti-PAK2 antibody at 1/500 dilution. |
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Fig3: ICC staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PAK2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PAK2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PAK2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PAK2 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.