Bcl-6 Recombinant Rabbit Monoclonal Antibody [JB18-48]
cat.: ET7107-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JB18-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bcl-6 aa 657-706 / 706. |
| Positive control: | Ramos cell lysate, Raji cell lysate, Daudi cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P41182 Human | P41183 Mouse |
| Alternative names: | B Cell Lymphoma 6 Protein B-cell CLL/lymphoma 6 B-cell lymphoma 5 protein B-cell lymphoma 6 protein transcript BCL5 BCL6A cys-his2 zinc finger transcription factor LAZ3 lymphoma-associated zinc finger gene on chromosome 3 Protein LAZ-3 ZBTB27 Zinc finger and BTB domain-containing protein 27 Zinc Finger Protein 51 zinc finger transcription factor BCL6S ZNF51 |
Images
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Fig1:
Western blot analysis of Bcl-6 on different lysates with Rabbit anti-Bcl-6 antibody (ET7107-80) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-80) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.