Flotillin 1 Recombinant Rabbit Monoclonal Antibody [JB19-45]
cat.: ET7107-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB19-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Flotillin 1 aa 378-427 / 427. |
| Positive control: | Neuro-2a cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, mouse lung tissue lysate, PC-3M cell lysates, rat brain tissue, human lung cancer tissue, human liver tissue, human spleen tissue, HUVEC. |
| Subcellular location: | Cell membrane. Endosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: O75955 Human | O08917 Mouse | Q9Z1E1 Rat |
| Alternative names: | FLOT 1 FLOT1 FLOT1_HUMAN Flotillin-1 Flotillin1 Integral membrane component of caveolae Reggie 2 |
Images
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Fig1:
Western blot analysis of Flotillin 1 on different lysates with Rabbit anti-Flotillin 1 antibody (ET7107-82) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-82) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.