Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat, Mouse |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JB19-45 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 47 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Flotillin 1 aa 378-427 / 427. |
Positive control: | PC-3M, rat brain tissue, human lung cancer tissue, human liver tissue, human spleen tissue, HUVEC. |
Subcellular location: | Cell membrane. Endosome. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
Uniprot #: | SwissProt: O75955 Human | O08917 Mouse | Q9Z1E1 Rat |
Alternative names: | FLOT 1 FLOT1 FLOT1_HUMAN Flotillin-1 Flotillin1 Integral membrane component of caveolae Reggie 2 |
Fig1: Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |