Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JB19-46 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 114 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human DSG1 aa 1,000-1,049 / 1,049. |
Positive control: | Human skin tissue, human colon cancer tissue, human uterus tissue, mouse skin tissue. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
IHC-P WB |
1:200-1:1,000 1:500 |
Uniprot #: | SwissProt: Q02413 Human | Q61495 Mouse |
Alternative names: | Cadherin family member 4 CDHF4 Desmoglein-1 Desmosomal glycoprotein 1 DG1 DGI DSG DSG1 DSG1_HUMAN EPKHE EPKHIA Pemphigus foliaceus antigen PPKS1 SPPK1 |
Fig1:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-DSG1 antibody (ET7107-83) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-83) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DSG1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DSG1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin. |