DSG1 Recombinant Rabbit Monoclonal Antibody [JB19-46]
cat.: ET7107-83
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JB19-46
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 114 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DSG1 aa 1,000-1,049 / 1,049.
Positive control: Human colon cancer tissue, human skin tissue, human uterus tissue, mouse skin tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  IHC-P
  WB

1:50-1:200
1:500
Uniprot #: SwissProt: Q02413 Human | Q61495 Mouse
Alternative names: Cadherin family member 4 CDHF4 Desmoglein-1 Desmosomal glycoprotein 1 DG1 DGI DSG DSG1 DSG1_HUMAN EPKHE EPKHIA Pemphigus foliaceus antigen PPKS1 SPPK1
Images
ET7107-83_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
ET7107-83_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-DSG1 antibody (ET7107-83) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-83) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-83_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
ET7107-83_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.