SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18]
cat.: ET7107-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JB49-18
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide of C terminal Human SNF5.
Positive control: Daudi cell lysate, PC-12 cell lysate, Human lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: Q12824 Human | Q9Z0H3 Mouse | Q4KLI0 Rat
Alternative names: BAF47 BRG1-associated factor 47 hSNF5 INI1 Integrase interactor 1 protein Malignant rhabdoid tumor suppressor RDT RTPS1 Sfh1p SMARCB1 SNF5 homolog SNF5_HUMAN SNF5L1 Snr1 Sucrose nonfermenting yeast homolog like 1 SWI/SNF complex component SNF5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 SWI10 Transcription factor TYE4 Transcription regulatory protein SNF5 TYE4
Images
ET7107-84_1.jpg Fig1: Western blot analysis of SNF5 on different lysates with Rabbit anti-SNF5 antibody (ET7107-84) at 1/1,000 dilution.

Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: Lane 1: 20 seconds; Lane 2-5: 40 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.