SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18]
cat.: ET7107-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB49-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide of C terminal Human SNF5. |
| Positive control: | Daudi cell lysates, mouse lymph node tissue lysate, rat kidney tissue, mouse colon tissue, SH-SY-5Y, human kidney tissue, mouse large intestine tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q12824 Human | Q9Z0H3 Mouse | Q4KLI0 Rat |
| Alternative names: | BAF47 BRG1-associated factor 47 hSNF5 INI1 Integrase interactor 1 protein Malignant rhabdoid tumor suppressor RDT RTPS1 Sfh1p SMARCB1 SNF5 homolog SNF5_HUMAN SNF5L1 Snr1 Sucrose nonfermenting yeast homolog like 1 SWI/SNF complex component SNF5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 SWI10 Transcription factor TYE4 Transcription regulatory protein SNF5 TYE4 |
Images
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Fig1: Western blot analysis of SNF5 on Daudi cell lysates and mouse lymph node tissue lysates using anti-SNF5 antibody at 1/500 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SNF5 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SNF5 antibody. Counter stained with hematoxylin. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SNF5 antibody (ET7107-84) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-84) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-SNF5 antibody (ET7107-84) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-84) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of SH-SY-5Y cells with SNF5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.