| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JB49-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide of C terminal Human SNF5. |
| Positive control: | Daudi cell lysate, PC-12 cell lysate, Human lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q12824 Human | Q9Z0H3 Mouse | Q4KLI0 Rat |
| Alternative names: | BAF47 BRG1-associated factor 47 hSNF5 INI1 Integrase interactor 1 protein Malignant rhabdoid tumor suppressor RDT RTPS1 Sfh1p SMARCB1 SNF5 homolog SNF5_HUMAN SNF5L1 Snr1 Sucrose nonfermenting yeast homolog like 1 SWI/SNF complex component SNF5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 SWI10 Transcription factor TYE4 Transcription regulatory protein SNF5 TYE4 |
|
Fig1:
Western blot analysis of SNF5 on different lysates with Rabbit anti-SNF5 antibody (ET7107-84) at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: Lane 1: 20 seconds; Lane 2-5: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |