Cubilin Recombinant Rabbit Monoclonal Antibody [JB42-13]
cat.: ET7107-88
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB42-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 399 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cubilin aa 3,376-3,521 / 3,623. |
| Positive control: | Human kidney tissue. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: O60494 Human |
| Alternative names: | 460 kDa receptor cubilin (intrinsic factor-cobalamin receptor) Cubilin Cubilin precursor Cubn CUBN_HUMAN IFCR Intestinal intrinsic factor receptor intrinsic factor B12-receptor Intrinsic factor-cobalamin receptor Intrinsic factor-vitamin B12 receptor megaloblastic anemia 1 MGA1 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cubilin antibody (ET7107-88) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-88) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.