Histone H4 (acetyl K16) Recombinant Rabbit Monoclonal Antibody [JB21-44]
cat.: ET7107-89
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JB21-44
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 11 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Histone H4 (acetyl K16) aa 1-50 / 103.
Positive control: SiHa cell lysates, Hela, SH-SY5Y, rat brain tissue, human tonsil tissue, human lung carcinoma tissue, mouse colon tissue.
Subcellular location: Chromosome, Nucleosome core, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:500-1:1,000
Uniprot #: SwissProt: P62805 Human | P62806 Mouse | P62804 Rat
Alternative names: Histone gene cluster 1, H4A Histone gene cluster 2, H4 dJ160A22.1 dJ160A22.2 dJ221C16.1 dJ221C16.9 FO108 H4 H4 histone family, member A H4 histone family, member B H4 histone family, member C H4 histone family, member D H4 histone family, member E H4 histone family, member G H4 histone family, member H H4 histone family, member I H4 histone family, member J H4 histone family, member K H4 histone family, member M H4 histone family, member N H4 histone, family 2 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4/p H4_HUMAN H4F2 H4F2iii H4F2iv H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK HIST1 cluster, H4A HIST1 cluster, H4B HIST1 cluster, H4D HIST2H4 Hist4 cluster, H4 Hist4h4 histone 1, H4a histone 1, H4c histone 1, H4d histone 1, H4f histone 1, H4h histone 1, H4i histone 1, H4j histone 1, H4k histone 1, H4l histone 2, H4a ......
Images
ET7107-89_1.jpg Fig1: Western blot analysis of Histone H4 (acetyl K16) on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7107-89_2.jpg Fig2: ICC staining of Histone H4 (acetyl K16) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-89_3.jpg Fig3: ICC staining of Histone H4 (acetyl K16) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-89_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Histone H4 (acetyl K16) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-89, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-89_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H4 (acetyl K16) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-89, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-89_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H4 (acetyl K16) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-89, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-89_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (acetyl K16) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-89, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-89_8.jpg Fig8: Flow cytometric analysis of Hela cells labeling Histone H4 (acetyl K16).

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-89, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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