Thrombomodulin Recombinant Rabbit Monoclonal Antibody [JB48-23]
cat.: ET7107-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB48-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thrombomodulin aa 526-575 / 575. |
| Positive control: | Human lung cancer tissue, human placenta tissue, A431, human lung tissue, human skin tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P07204 Human |
| Alternative names: | AHUS 6 AHUS6 BDCA 3 BDCA3 CD 141 CD141 CD141 antigen Fetomodulin Thbd THPH12 THRM Thrombomodulin TM TRBM_HUMAN |
Images
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Fig1: Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Thrombomodulin antibody (ET7107-92) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-92) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Thrombomodulin antibody (ET7107-92) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-92) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.