CD46 Recombinant Rabbit Monoclonal Antibody [JB25-49]
cat.: ET7107-94
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB25-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD46 aa 31-80 / 392. |
| Positive control: | SiHa cell lysate, PC-3M cell lysate, PC-3M, HUVEC, human colon carcinoma tissue, human colon tissue, human breast tissue, human placenta tissue, rat kidney tissue, human prostate tissue. |
| Subcellular location: | Acrosome inner membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:400-1:800 |
| Uniprot #: | SwissProt: P15529 Human | Q9Z0M4 Rat |
| Alternative names: | AHUS2 Antigen defined by monoclonal TRA 2 10 Antigen identified by monoclonal TRA 2 10 CD46 CD46 antigen CD46 antigen complement regulatory protein CD46 molecule CD46 molecule complement regulatory protein Complement membrane cofactor protein MCP MCP_HUMAN Measles virus receptor membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) Membrane cofactor protein MGC26544 MIC10 TLX TRA2.10 Trophoblast leucocyte common antigen Trophoblast leukocyte common antigen Trophoblast lymphocyte cross reactive antigen |
Images
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Fig1:
Western blot analysis of CD46 on different lysates with Rabbit anti-CD46 antibody (ET7107-94) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CD46 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 55-70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-94) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.