CD46 Recombinant Rabbit Monoclonal Antibody [JB25-49]
cat.: ET7107-94
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JB25-49
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD46 aa 31-80 / 392.
Positive control: SiHa cell lysate, PC-3M cell lysate, PC-3M, HUVEC, human colon carcinoma tissue, human colon tissue, human breast tissue, human placenta tissue, rat kidney tissue, human prostate tissue.
Subcellular location: Acrosome inner membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:2,000
1:50-1:200
1:50-1:800
Uniprot #: SwissProt: P15529 Human | Q9Z0M4 Rat
Alternative names: AHUS2 Antigen defined by monoclonal TRA 2 10 Antigen identified by monoclonal TRA 2 10 CD46 CD46 antigen CD46 antigen complement regulatory protein CD46 molecule CD46 molecule complement regulatory protein Complement membrane cofactor protein MCP MCP_HUMAN Measles virus receptor membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) Membrane cofactor protein MGC26544 MIC10 TLX TRA2.10 Trophoblast leucocyte common antigen Trophoblast leukocyte common antigen Trophoblast lymphocyte cross reactive antigen
Images
ET7107-94_1.jpg Fig1: Western blot analysis of CD46 on different lysates with Rabbit anti-CD46 antibody (ET7107-94) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD46 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 55-70 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-94) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-94_2.jpg Fig2: Western blot analysis of CD46 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: PC-3M cell lysate
ET7107-94_3.jpg Fig3: ICC staining of CD46 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-94_4.jpg Fig4: ICC staining of CD46 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-94_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-94_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-94_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-94_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-94_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD46 antibody (ET7107-94) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-94) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.