CAMKIV Recombinant Rabbit Monoclonal Antibody [JB33-13]
cat.: ET7107-96
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JB33-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CAMKIV aa 360-473 / 473.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate, Hela, SH-SY5Y, 293T, rat brain tissue, rat cerebellum tissue, mouse brain tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q16566 Human | P08414 Mouse | P13234 Rat
Alternative names: Brain Ca(2+) calmodulin dependent protein kinase type 4 Brain Ca(2+) calmodulin dependent protein kinase type IV Brain Ca++-calmodulin dependent protein kinase type IV Calcium / calmodulin dependent protein kinase type 4 catalytic chain Calcium / calmodulin dependent protein kinase type IV catalytic chain Calcium/calmodulin dependent protein kinase IV Calcium/calmodulin dependent protein kinase type IV Calcium/calmodulin-dependent protein kinase type IV CAM kinase 4 CAM kinase GR CAM kinase IV CAM kinase-GR CaMK 4 CAMK GR CaMK IV Camk4 CaMKGR IV KCC4_HUMAN MGC36771
Images
ET7107-96_1.jpg Fig1: Western blot analysis of CAMKIV on different lysates with Rabbit anti-CAMKIV antibody (ET7107-96) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD CAMKIV cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-96_2.jpg Fig2: Western blot analysis of CAMKIV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
ET7107-96_3.jpg Fig3: ICC staining of CAMKIV in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-96_4.jpg Fig4: ICC staining of CAMKIV in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-96_5.jpg Fig5: ICC staining of CAMKIV in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-96_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-96, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-96_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CAMKIV antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-96, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-96_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-96, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-96_9.jpg Fig9: Flow cytometric analysis of CAMKIV was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-96, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.