Arp3 Recombinant Rabbit Monoclonal Antibody [JB33-44]
cat.: ET7107-97
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JB33-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Arp3 aa 308-418 / 418. |
| Positive control: | A431 cell lysate, mouse placenta tissue lysate, mouse thymus tissue lysate, HUVEC, LOVO, SiHa, rat kidney tissue, human placenta tissue, human stomach cancer tissue, mouse kidney tissue, HL-60. |
| Subcellular location: | Cell projection, Cytoplasm, Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P61158 Human | Q99JY9 Mouse | Q4V7C7 Rat |
| Alternative names: | Actin like protein 3 Actin related protein 3 ACTR3 ARP3 actin related protein 3 homolog (yeast) |
Images
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Fig1:
Western blot analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution. Positive control: Lane 1: A431 cell lysate Lane 2: Mouse placenta tissue lysate Lane 3: Mouse thymus tissue lysate |
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Fig2: ICC staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Arp3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Arp3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Arp3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.