Anti-U1A antibody [JB33-48]
cat.: ET7107-98
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC-P, IP
Clonality: Monoclonal
Clone number: JB33-48
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human U1A .
Positive control: Rat brain tissue lysate, K562, A431, mouse kidney tissue lysate, 293T, SH-SY-5Y, SiHa, human lung cancer tissue, mouse liver tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  IP

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P09012 Human | Q62189 Mouse
Alternative names: fc19d01 Mud1 small nuclear ribonucleoprotein polypeptide A snRNP A snRNP protein A SNRPA SNRPA_HUMAN U1 small nuclear ribonucleoprotein A U1 small nuclear RNP specific A U1 snRNP A U1 snRNP specific protein A U1-A U1A wu:fc19d01 zgc:77810
Images
ET7107-98_1.jpg Fig1: Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: K562
Lane 3: A431
Lane 4: Mouse kidney
ET7107-98_2.jpg Fig2: ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-98_3.jpg Fig3: ICC staining U1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-98_4.jpg Fig4: ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-98_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-U1A antibody. Counter stained with hematoxylin.
ET7107-98_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-U1A antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.