Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86]
cat.: ET7108-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG33-86 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Glucose 6 phosphate isomerase aa 1-128 / 558. |
| Positive control: | A549 cell lysates, HepG2 cell lysates, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue, A549. |
| Subcellular location: | Cytoplasm. Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P06744 Human |
| Alternative names: | AMF Aurocrine motility factor Autocrine motility factor DKFZp686C13233 EC 5.3.1.9 G6PI_HUMAN Glucose phosphate isomerase Glucose-6-phosphate isomerase GNPI GPI Gpi1 Hexose monophosphate isomerase Hexosephosphate isomerase Neuroleukin NLK Oxoisomerase PGI PHI Phosphoglucose isomerase Phosphohexomutase Phosphohexose isomerase Phosphosaccharomutase SA 36 SA-36 SA36 |
Images
|
Fig1:
Western blot analysis of Glucose 6 phosphate isomerase on different lysates with Rabbit anti-Glucose 6 phosphate isomerase antibody (ET7108-01) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glucose 6 phosphate isomerase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-01) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of Glucose 6 phosphate isomerase on A549 cell lysates and HepG2 cell lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution. |
|
Fig3: ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. |
|
Fig8: Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.