CYP26A1 Recombinant Rabbit Monoclonal Antibody [JG33-98]
cat.: ET7108-02
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG33-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CYP26A1 aa 181-470 / 497. |
| Positive control: | HepG2 cell lysates, 293 cell lysate, human liver tissue lysate, human liver tissue, human pancreas tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane, Microsome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: O43174 Human | Q8VIL0 Rat |
| Alternative names: | CP26 CP26A_HUMAN CYP26 CYP26A 1 cyp26a1 Cytochrome P450 26A1 Cytochrome P450 family 26 subfamily A polypeptide 1 Cytochrome P450 retinoic acid-inactivating 1 cytochrome P450, family 26, subfamily A, polypeptide 1 cytochrome P450, subfamily XXVIA, polypeptide 1 Cytochrome P450RAI hP450RAI OTTHUMP00000020102 OTTHUMP00000020103 P450 P450 retinoic acid inactivating 1 P450RAI P450RAI1 Retinoic acid 4 hydroxylase Retinoic acid 4-hydroxylase Retinoic acid metabolizing cytochrome Retinoic acid-metabolizing cytochrome |
Images
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Fig1: Western blot analysis of CYP26A1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CYP26A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Human liver tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CYP26A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CYP26A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.