ERp29 Recombinant Rabbit Monoclonal Antibody [JG34-42]
cat.: ET7108-03
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG34-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ERp29 aa 1-142 / 261. |
| Positive control: | THP-1 cell lysate, F9, PMVEC, SiHa, rat testis tissue, human thyroid gland tissue, human colon cancer tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P30040 Human | P57759 Mouse | P52555 Rat |
| Alternative names: | C12orf8 Chromosome 12 open reading frame 8 Endoplasmic reticulum lumenal protein ERp28 Endoplasmic reticulum protein 29 Endoplasmic reticulum protein 29 isoform 1 Endoplasmic reticulum protein ERp29 Endoplasmic reticulum resident protein 28 Endoplasmic reticulum resident protein 29 Endoplasmic reticulum resident protein 31 Epididymis secretory protein Li 107 ERp28 ERP29_HUMAN ERp31 HEL S 107 PDI-DB PDIA9 Protein disulfide isomerase family A member 9 |
Images
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Fig1:
Western blot analysis of ERp29 on different lysates with Rabbit anti-ERp29 antibody (ET7108-03) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-ERp29 KD cell lysate (10 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-03) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ERp29 on THP-1 cell lysate using anti-ERp29 antibody at 1/500 dilution. |
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Fig3: ICC staining ERp29 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ERp29 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining ERp29 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp29 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-ERp29 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERp29 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.