Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]
cat.: ET7108-04
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, VIVIT |
| Clonality: | Monoclonal |
| Clone number: | JG34-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Gephyrin aa 5-95 / 736. |
| Positive control: | SK-Br-3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse brain tissue. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:400 |
| Uniprot #: | SwissProt: Q9NQX3 Human | Q8BUV3 Mouse | Q03555 Rat |
| Alternative names: | Domain E Domain G GEPH GEPH_HUMAN GPH GPHN GPHRYN KIAA1385 Molybdopterin molybdenumtransferase MPT adenylyltransferase MPT Mo-transferase |
Images
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Fig1:
Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (15 µg/Lane) Lane 2: Neuro-2a cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Mouse liver tissue lysate (30 µg/Lane) Lane 6: Mouse kidney tissue lysate (30 µg/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.