PARP2 Recombinant Rabbit Monoclonal Antibody [JG34-56]
cat.: ET7108-05
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JG34-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PARP2 aa 484-583 / 583. |
| Positive control: | HCT 116 cell lysate, HeLa cell lysate. |
| Subcellular location: | Chromosome, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9UGN5 Human |
| Alternative names: | ADP ribosyltransferase like 2 ADP-ribosyltransferase diphtheria toxin-like 2 ADPRT 2 ADPRT-2 ADPRT2 ADPRTL 2 ADPRTL 3 ADPRTL2 ADPRTL3 ARTD2 hPARP 2 hPARP-2 hPARP2 NAD(+) ADP ribosyltransferase 2 NAD(+) ADP-ribosyltransferase 2 pADPRT 2 pADPRT-2 pADPRT2 PARP 2 PARP-2 PARP2 PARP2_HUMAN Poly (ADP ribose) polymerase family member 2 Poly (ADP ribosyl) transferase like 2 poly (ADP-ribose) polymerase 2 Poly [ADP ribose] synthetase 2 Poly [ADP-ribose] polymerase 2 Poly(ADP ribose) synthetase Poly[ADP-ribose] synthase 2 |
Images
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Fig1:
Western blot analysis of PARP2 on different lysates with Rabbit anti-PARP2 antibody (ET7108-05) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PARP2 on different lysates with Rabbit anti-PARP2 antibody (ET7108-05) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si PARP2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa Observed band size: 55 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.