ELMO1 Recombinant Rabbit Monoclonal Antibody [JG34-59]
cat.: ET7108-06
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JG34-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ELMO1 aa 678-727 / 727. |
| Positive control: | HeLa cell lysate, mouse lung tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate, rat spleen tissue, mouse spleen tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q92556 Human | Q8BPU7 Mouse | D3ZY46 Rat |
| Alternative names: | CED 12 Ced 12 homolog 1 Ced 12 homolog CED-12 CED12 Ced12 homolog 1 Ced12 homolog ELMO 1 ELMO-1 Elmo1 ELMO1_HUMAN Engulfment and cell motility 1 Engulfment and cell motility protein 1 KIAA0281 MGC126406 Protein ced-12 homolog |
Images
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Fig1:
Western blot analysis of ELMO1 on different lysates with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/500 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (40 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-06) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-ELMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ELMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of ELMO1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-06, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.