ELMO1 Recombinant Rabbit Monoclonal Antibody [JG34-59]
cat.: ET7108-06
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG34-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ELMO1 aa 678-727 / 727. |
| Positive control: | SH-SY5Y cell lysate, Raji cell lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat spleen tissue lysate, human appendix tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q92556 Human | Q8BPU7 Mouse | D3ZY46 Rat |
| Alternative names: | CED 12 Ced 12 homolog 1 Ced 12 homolog CED-12 CED12 Ced12 homolog 1 Ced12 homolog ELMO 1 ELMO-1 Elmo1 ELMO1_HUMAN Engulfment and cell motility 1 Engulfment and cell motility protein 1 KIAA0281 MGC126406 Protein ced-12 homolog |
Images
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Fig1:
Western blot analysis of ELMO1 on different lysates with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Raji cell lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-06) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
ELMO1 was immunoprecipitated from 0.2 mg Raji cell lysate with ET7108-06 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET7108-06 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: ET7108-06 IP in Raji cell lysate Lane 3: Rabbit IgG instead of ET7108-06 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.