Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]
cat.: ET7108-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JG34-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Nuf2 aa 1-108 / 464. |
| Positive control: | K562 cell lysate, PC-3M cell lysate, THP-1 cell lysate, A431. |
| Subcellular location: | Centromere, Chromosome, Kinetochore, Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9BZD4 Human |
| Alternative names: | Cancer/testis antigen 106 CDCA 1 CDCA1 Cell division cycle associated 1 Cell division cycle associated protein 1 Cell division cycle-associated protein 1 CT106 hNuf 2 hNuf2 hNuf2R hsNuf2 Kinetochore protein Nuf2 Nuf 2 NUF2 NUF2 NDC80 kinetochore complex component NUF2 NDC80 kinetochore complex component homolog NUF2, S. cerevisiae, homolog of NUF2_HUMAN NUF2R |
Images
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Fig1:
Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution. Positive control: Line 1: K562 cell lysate Line 2: PC-3M cell lysate Line 3: THP-1 cell lysate |
|
Fig2: Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.