Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]
cat.: ET7108-07
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: JG34-64
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Nuf2 aa 1-108 / 464.
Positive control: K562 cell lysate, PC-3M cell lysate, THP-1 cell lysate, A431.
Subcellular location: Centromere, Chromosome, Kinetochore, Nucleus.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q9BZD4 Human
Alternative names: Cancer/testis antigen 106 CDCA 1 CDCA1 Cell division cycle associated 1 Cell division cycle associated protein 1 Cell division cycle-associated protein 1 CT106 hNuf 2 hNuf2 hNuf2R hsNuf2 Kinetochore protein Nuf2 Nuf 2 NUF2 NUF2 NDC80 kinetochore complex component NUF2 NDC80 kinetochore complex component homolog NUF2, S. cerevisiae, homolog of NUF2_HUMAN NUF2R
Images
ET7108-07_1.jpg Fig1: Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.
Positive control:
Line 1: K562 cell lysate
Line 2: PC-3M cell lysate
Line 3: THP-1 cell lysate
ET7108-07_2.jpg Fig2: Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.