USP9x Recombinant Rabbit Monoclonal Antibody [JG35-11]
cat.: ET7108-08
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG35-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 290 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human USP9x aa 1-118 / 2,554.
Positive control: SiHa cell lysate, A549 cell lysate, 293 cell lysate, mouse colon tissue lysate, 293T, F9, SiHa, human tonsil tissue, mouse fallopian tube tissue.
Subcellular location: Cell projection, Cytoplasm, Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q93008 Human | P70398 Mouse | D3ZC84 Rat
Alternative names: Deubiquitinating enzyme FAF X Deubiquitinating enzyme FAF-X DFFRX Drosophila fat facets related X linked FAF Fafl Fam Fat facets homolog Fat facets in mammals Fat facets protein related X linked Fat facets protein related, X-linked Fat facets protein-related hFAM MRX99 Probable ubiquitin carboxyl terminal hydrolase FAF X Probable ubiquitin carboxyl-terminal hydrolase FAF-X Ubiquitin carboxyl-terminal hydrolase FAM Ubiquitin specific peptidase 9 X linked Ubiquitin specific peptidase 9, X-linked Ubiquitin specific processing protease FAF X Ubiquitin specific protease 9 X chromosome Ubiquitin thioesterase FAF X Ubiquitin thiolesterase FAF X Ubiquitin thiolesterase FAF-X Ubiquitin-specific protease 9 Ubiquitin-specific-processing protease FAF-X USP9 (gene name) Usp9x USP9X_HUMAN Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila) X chromosome X-linked
Images
ET7108-08_1.jpg Fig1: Western blot analysis of USP9x on different lysates with Rabbit anti-USP9x antibody (ET7108-08) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si USP9x cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 290 kDa
Observed band size: 290 kDa

Exposure time: 18 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-08) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-08_2.jpg Fig2: Western blot analysis of USP9x on different lysates with Rabbit anti-USP9x antibody (ET7108-08) at 1/500 dilution.

Lane 1: SiHa cell lysate (10 µg/Lane)
Lane 2: A549 cell lysate (10 µg/Lane)
Lane 3: 293 cell lysate (10 µg/Lane)
Lane 4: Mouse colon tissue lysate (20 µg/Lane)

Predicted band size: 290 kDa
Observed band size: 290 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
ET7108-08_3.jpg Fig3: ICC staining of USP9x in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-08, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-08_4.jpg Fig4: ICC staining of USP9x in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-08, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-08_5.jpg Fig5: ICC staining of USP9x in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-08, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-08_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-USP9x antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-08_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9x antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-08_8.jpg Fig8: Flow cytometric analysis of USP9x was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-08, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.