eIF4A3 Recombinant Rabbit Monoclonal Antibody [JG35-33]
cat.: ET7108-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JG35-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal Human eIF4A3 . |
| Positive control: | SiHa cell lysates, LOVO, MCF-7, SiHa, human tonsil tissue, human colon carcinoma, human prostate cancer tissue, mouse skin tissue, rat skin tissue, human lymph nodes tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Spliceosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P38919 Human | Q91VC3 Mouse | Q3B8Q2 Rat |
| Alternative names: | ATP-dependent RNA helicase DDX48 ATP-dependent RNA helicase eIF4A-3 DDX48 DEAD box protein 48 eIF-4A-III eIF4A-III EIF4A3 eIF4AIII Eukaryotic initiation factor 4A-III Eukaryotic initiation factor 4A-like NUK-34 Eukaryotic translation initiation factor 4A isoform 3 hNMP 265 IF4A3_HUMAN NMP 265 NMP265 Nuclear matrix protein 265 NUK34 |
Images
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Fig1: Western blot analysis of eIF4A3 on SiHa cell lysates using anti-eIF4A3 at 1/500 dilution. |
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Fig2: ICC staining eIF4A3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining eIF4A3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining eIF4A3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eIF4A3 antibody (ET7108-11) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-11) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of MCF-7 cells with eIF4A3 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-eIF4A3 antibody (ET7108-11) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-11) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig11:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-eIF4A3 antibody (ET7108-11) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-11) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.