Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
cat.: ET7108-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC, IP, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG37-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 126 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Pumilio 1 aa 79-223 . |
| Positive control: | SiHa cell lysate, NCCIT cell lysate, 293T, SiHa, human colon tissue, human appendix tissue, mouse kidney tissue, mouse small intestine tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q14671 Human | Q80U78 Mouse |
| Alternative names: | HsPUM KIAA0099 PUM 1 PUM L1 Pum1 PUM1_HUMAN PUMH 1 PUMH PUMH1 pumilio homolog 1 (Drosophila) Pumilio homolog 1 Pumilio-1 Pumilio1 PUML 1 PUML1 |
Images
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Fig1:
Western blot analysis of Pumilio 1 on different lysates with Rabbit anti-Pumilio 1 antibody (ET7108-13) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-13, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 126 kDa Observed band size: 126 kDa |
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Fig2: ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.