| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG35-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 101 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CHD1L aa 680-820 / 897. |
| Positive control: | HepG2 cell lysate , human tonsil tissue, human colon cancer tissue, mouse testis tissue, HepG2. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q86WJ1 Human | Q9CXF7 Mouse |
| Alternative names: | ALC1 Amplified in liver cancer 1 Amplified in liver cancer protein 1 chd1l CHD1L_HUMAN CHDL Chromodomain helicase DNA binding protein 1 like Chromodomain-helicase-DNA-binding protein 1-like FLJ22530 |
|
Fig1: Western blot analysis of CHD1L on HepG2 cell lysate using anti-CHD1L antibody at 1/1,000 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig5: Flow cytometric analysis of HepG2 cells with CHD1L antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |