Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JG35-44 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 101 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human CHD1L aa 680-820 / 897. |
Positive control: | HepG2 cell lysate , human tonsil tissue, human colon cancer tissue, mouse testis tissue, HepG2. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
Uniprot #: | SwissProt: Q86WJ1 Human | Q9CXF7 Mouse |
Alternative names: | ALC1 Amplified in liver cancer 1 Amplified in liver cancer protein 1 chd1l CHD1L_HUMAN CHDL Chromodomain helicase DNA binding protein 1 like Chromodomain-helicase-DNA-binding protein 1-like FLJ22530 |
Fig1: Western blot analysis of CHD1L on HepG2 cell lysate using anti-CHD1L antibody at 1/1,000 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
Fig5: Flow cytometric analysis of HepG2 cells with CHD1L antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |