Syntrophin alpha 1 Recombinant Rabbit Monoclonal Antibody [JG35-62]
cat.: ET7108-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG35-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Syntrophin alpha 1 aa 1-49 / 505. |
| Positive control: | SiHa cell lysate, NIH/3T3 cell lysate, human skeletal muscle tissue, rat skeletal muscle tissue, mouse colon tissue, human colon tissue, LOVO. |
| Subcellular location: | Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q13424 Human | Q61234 Mouse Entrez Gene: 362242 Rat |
| Alternative names: | 59 kDa dystrophin-associated protein A1 acidic component 1 Acidic alpha 1 syntrophin Alpha 1 syntrophin Alpha-1-syntrophin dJ1187J4.5 Dystrophin associated protein A1 59kDa acidic component LQT12 OTTHUMP00000030650 Pro-TGF-alpha cytoplasmic domain-interacting protein 1 SNT1 Snta1 SNTA1_HUMAN Syntrophin 1 Syntrophin-1 TACIP1 |
Images
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Fig1:
Western blot analysis of Syntrophin alpha 1 on different lysates with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling Syntrophin alpha 1 with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7108-15, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.