TREX1 Recombinant Rabbit Monoclonal Antibody [JG35-71]
cat.: ET7108-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JG35-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TREX1 aa 110-314 / 314.
Positive control: A431 cell lysate, SK-Br-3 cell lysate, Hela, LOVO, SiHa, human colon tissue, human prostate carcinoma tissue.
Subcellular location: Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q9NSU2 Human
Alternative names: 3' 5' exonuclease TREX1 3' repair exonuclease 1 AGS1 AGS5 CRV Deoxyribonuclease III, dnaQ/mutD (E. coli) like DKFZp434J0310 DNase III DRN3 HERNS Three prime repair exonuclease 1 TREX1
Images
ET7108-16_1.jpg Fig1: Western blot analysis of TREX1 on A431 cell lysates with Rabbit anti-TREX1 antibody (ET7108-16) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 1 minute 50 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-16_2.jpg Fig2: ICC staining of TREX1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-16_3.jpg Fig3: ICC staining of TREX1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-16_4.jpg Fig4: ICC staining of TREX1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-16_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TREX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-16_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-TREX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-16_7.jpg Fig7: Flow cytometric analysis of A431 cells labeling TREX1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-16, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7108-16_8.jpg Fig8: TREX1 was immunoprecipitated from 0.2 mg A431 cell lysate with ET7108-16 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7108-16 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: A431 cell lysate (input)
Lane 2: ET7108-16 IP in A431 cell lysate
Lane 3: Rabbit IgG instead of ET7108-16 in A431 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.