hnRNP Q Recombinant Rabbit Monoclonal Antibody [JG35-72]
cat.: ET7108-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IHC-P, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JG35-72
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human hnRNP Q aa 574-623 / 623.
Positive control: K562 cell lysates, rat brain tissue lysates, LOVO, SiHa, rat testis tissue, human thyroid tissue, human breast tissue, human kidney tissue.
Subcellular location: Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus, Spliceosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O60506 Human | Q7TP47 Rat | Q7TMK9 Mouse
Alternative names: cytoplasmic RNA-interacting protein dJ3J17.2 Glycine and tyrosine rich RNA binding protein Glycine- and tyrosine-rich RNA-binding protein GRY RBP GRY-RBP GRYRBP Heterogeneous nuclear ribonucleoprotein Q hnRNP Q HNRPQ HNRPQ_HUMAN HNRPQ1 NS1 associated protein 1 NS1-associated protein 1 NSAP1 pp68 RP1 3J17.2 Synaptotagmin binding cytoplasmic RNA interacting protein Synaptotagmin-binding Syncrip
Images
ET7108-17_1.jpg Fig1: Western blot analysis of hnRNP Q on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-17_2.jpg Fig2: Western blot analysis of hnRNP Q on rat brain tissue lysates with Rabbit anti-hnRNP Q antibody (ET7108-17) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-17_3.jpg Fig3: ICC staining of hnRNP Q in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-17_4.jpg Fig4: ICC staining of hnRNP Q in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-17_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-hnRNP Q antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-17_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-hnRNP Q antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-17_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP Q antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-17_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-hnRNP Q antibody (ET7108-17) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.