PRPF8 Recombinant Rabbit Monoclonal Antibody [JG57-38]
cat.: ET7108-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JG57-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 274 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PRPF8 aa 2,200-2,335 / 2,335.
Positive control: SiHa cell lysate, A549 cell lysate, 293T, LOVO, SiHa, rat brain tissue, human colon tissue, human prostate carcinoma tissue, mouse testis tissue, A549.
Subcellular location: Nucleus. Spliceosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q6P2Q9 Human | Q99PV0 Mouse | G3V6H2 Rat
Alternative names: 220 kDa U5 snRNP specific protein 220 kDa U5 snRNP-specific protein Apoptosis regulated protein 1 Apoptosis regulated protein 2 HPRP8 p220 Pre mRNA processing factor 8 Pre mRNA-processing factor 8, S. cerevisiae, homolog of Pre-mRNA-processing-splicing factor 8 Precursor mRNA processing protein PRP8 PRP8 homolog PRP8 pre mRNA processing factor 8 homolog PRP8_HUMAN PRPC8 Prpf8 Retinitis pigmentosa 13 (autosomal dominant) RP13 SNRNP220 Splicing factor Prp8 U5 snRNP specific protein U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Images
ET7108-18_1.jpg Fig1: Western blot analysis of PRPF8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
ET7108-18_2.jpg Fig2: ICC staining of PRPF8 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-18_3.jpg Fig3: ICC staining of PRPF8 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-18_4.jpg Fig4: ICC staining of PRPF8 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-18_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PRPF8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-18_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PRPF8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-18_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-PRPF8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-18_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PRPF8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-18_9.jpg Fig9: Flow cytometric analysis of PRPF8 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-18, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
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