PON2 Recombinant Rabbit Monoclonal Antibody [JG35-80]
cat.: ET7108-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG35-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human PON2 aa 160-354 / 354. |
| Positive control: | A549 cell lysate, HCT116 cell lysate, human colon carcinoma tissue, human appendix tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:2,000 1:10-1:50 1:50-1:200 |
| Uniprot #: | SwissProt: Q15165 Human | Q62086 Mouse | Q6AXM8 Rat |
| Alternative names: | A esterase 2 A-esterase 2 Aromatic esterase 2 Paraoxonase 2 Paraoxonase nirs PON 2 PON2 PON2_HUMAN Serum aryldialkylphosphatase 2 Serum paraoxonase/arylesterase 2 |
Images
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Fig1:
Western blot analysis of PON2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: HCT116 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PON2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-PON2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.