SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88]
cat.: ET7108-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: JG35-88
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SAE1 aa 201-249 / 346.
Positive control: SiHa cell lysate, K562 cell lysate, 293 cell lysate, A549.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q9UBE0 Human
Alternative names: Activator of SUMO1 AOS1 HSPC140 Sae1 SAE1_HUMAN Sentrin/SUMO activating protein AOS1 SUA1 SUMO 1 activating enzyme E1 N subunit SUMO 1 activating enzyme subunit 1 SUMO-activating enzyme subunit 1 Ubiquitin like protein SUMO1 activating enzyme Ubiquitin-like 1-activating enzyme E1A UBL E1A UBLE1A
Images
ET7108-22_1.jpg Fig1: Western blot analysis of SAE1 on different lysates with Rabbit anti-SAE1 antibody (ET7108-22) at 1/1,000 dilution.

Lane 1: SiHa cell lysates
Lane 2: K562 cell lysates
Lane 3: 293 cell lysates

Lysates/proteins at 10 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-22) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7108-22_2.jpg Fig2: Flow cytometric analysis of A549 cells with SAE1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.