KAT8 Recombinant Rabbit Monoclonal Antibody [JG36-05]
cat.: ET7108-23
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG36-05
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human KAT8 aa 310-458 / 458.
Positive control: HEK-293 cell lysate, HepG2 cell lysate, PC-3M cell lysate, MCF-7, SH-SY-5Y, SiHa, human cervix tissue, human cervix cancer tissue, rat cervix tissue, K562.
Subcellular location: Chromosome. Nucleus.
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC
  WB
1:50-1:200
1:50-1:200
1:50-1:100
1:1,000
Uniprot #: SwissProt: Q9H7Z6 Human | Q9D1P2 Mouse | Q5XI06 Rat
Alternative names: Histone acetyltransferase KAT8 Histone acetyltransferase MYST1 hMOF K(lysine) acetyltransferase 8 KAT 8 Lysine acetyltransferase 8 MOF MOZ MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 MYST 1 MYST histone acetyltransferase 1 myst protein 1 MYST-1 MYST1 MYST1_HUMAN Ortholog of Drosophila males absent on the first (MOF) Probable histone acetyltransferase MYST1 SAS2 and TIP60 protein 1 SAS2 SAS3 TIP60 protein 1 YBF2 YBF2/SAS3 ZC2HC8
Images
ET7108-23_1.jpg Fig1: Western blot analysis of KAT8 on different lysates with Rabbit anti-KAT8 antibody (ET7108-23) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC-3M cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-23) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-23_2.jpg Fig2: Immunocytochemistry analysis of MCF-7 cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-23_3.jpg Fig3: Immunocytochemistry analysis of SH-SY-5Y cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-23_4.jpg Fig4: Immunocytochemistry analysis of SiHa cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-23_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-23_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-23_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-23_8.jpg Fig8: Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.