KAT8 Recombinant Rabbit Monoclonal Antibody [JG36-05]
cat.: ET7108-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG36-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KAT8 aa 310-458 / 458. |
| Positive control: | MCF-7, SH-SY-5Y, SiHa, human cervix tissue, human cervix cancer tissue, rat cervix tissue, K562. |
| Subcellular location: | Chromosome. Nucleus. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q9H7Z6 Human | Q9D1P2 Mouse | Q5XI06 Rat |
| Alternative names: | Histone acetyltransferase KAT8 Histone acetyltransferase MYST1 hMOF K(lysine) acetyltransferase 8 KAT 8 Lysine acetyltransferase 8 MOF MOZ MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 MYST 1 MYST histone acetyltransferase 1 myst protein 1 MYST-1 MYST1 MYST1_HUMAN Ortholog of Drosophila males absent on the first (MOF) Probable histone acetyltransferase MYST1 SAS2 and TIP60 protein 1 SAS2 SAS3 TIP60 protein 1 YBF2 YBF2/SAS3 ZC2HC8 |
Images
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Fig1:
Immunocytochemistry analysis of MCF-7 cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Immunocytochemistry analysis of SH-SY-5Y cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.