CENPC Recombinant Rabbit Monoclonal Antibody [JG36-15]
cat.: ET7108-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG36-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 107 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CENPC aa 360-540 / 943. |
| Positive control: | Daudi cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Nucleus, kinetochore, centromere. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50 |
| Uniprot #: | SwissProt: Q03188 Human | P49452 Mouse Entrez Gene: 305270 Rat |
| Alternative names: | CENP-C 1 CENP-C CENPC_HUMAN CENPC1 Centromere autoantigen C Centromere autoantigen C1 Centromere protein C 1 Centromere protein C hcp-4 ICEN7 Interphase centromere complex protein 7 MIF2 |
Images
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Fig1:
Western blot analysis of CENPC on different lysates with Rabbit anti-CENPC antibody (ET7108-24) at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: HL-60 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (20 µg/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CENPC antibody (ET7108-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CENPC antibody (ET7108-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.