Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat, Mouse |
Applications: | WB, IP, IHC-P, FC, IF-Cell |
Clonality: | Monoclonal |
Clone number: | JG61-36 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 22 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human NDUFB8 aa 1-186 / 186. |
Positive control: | 293 cell lysate, A549 cell lysate, rat spleen tissue lysate, Hela cell lysate, rat heart tissue, human liver carcinoma tissue, human kidney tissue, human small intestine tissue, Hela. |
Subcellular location: | Mitochondrion. |
Recommended Dilutions:
WB IP IHC-P FC IF-Cell |
1:500-1:2,000 1:10-1:50 1:50-1:200 1:50-1:100 1:50 |
Uniprot #: | SwissProt: O95169 Human | Q9D6J5 Mouse |
Alternative names: | ASHI CI-ASHI Complex I ASHI subunit Complex I-ASHI mitochondrial NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial NADH-ubiquinone oxidoreductase ASHI subunit NDUB8_HUMAN NDUFB8 |
Fig1:
Western blot analysis of NDUFB8 on different lysates with Rabbit anti-NDUFB8 antibody (ET7108-25) at 1/500 dilution. Lane 1: 293 cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: Rat spleen tissue lysate (20 µg/Lane) Lane 4: Hela cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 19 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-25) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-NDUFB8 antibody (ET7108-25) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-25) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NDUFB8 antibody (ET7108-25) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-25) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFB8 antibody (ET7108-25) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-25) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-NDUFB8 antibody (ET7108-25) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-25) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of NDUFB8 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-25, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |